Figure 1.

High-throughput screening of the National Cancer Institute Developmental Therapeutics Program’s 3095 small molecule plated sets from the Open Chemical Repository Collection. (A) Scattered plot summarizing the results of the electrochemiluminescent integrase (IN) ST screening assay. Experiments were performed in a 96-well plate format, where each plate harbored eight control points with DMSO (IN alone) and a dose response of MA-DKA (also known as 118-D-24) over eight concentrations. Altogether, the screen necessitated 39 assay plates. Open diamonds represent the three stilbene disulfonic acid derivatives identified by the screen. (B) Chemical structure of the two top hits and the three stilbene disulfonic acid derivatives.