Figure 2.

Effects of 1 on the expression of (A) TLR4, (B) MyD88, (C) iNOS, (D) COX-2, (E) IL-6, (F) TNF-α and (G) NF-κB. RAW 264.7 cells were incubated with the indicated concentrations of 1 (2.5, 5 or 10 μM) and LPS (1 μg/mL) for 24 h, as determined by RT-PCR assay. GAPDH was used as the internal control. Values are representative of three independent experiments. ^## p < 0.01, ^### p < 0.005 vs. control cells. * p < 0.05, ** p < 0.01, *** p < 0.005 vs. LPS-induced cells.