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. 2019 Oct 16;24(20):3731. doi: 10.3390/molecules24203731

Figure 5.

Figure 5

Effect of 1 on NO and ROS production in LPS-induced RAW 264.7 macrophages. (A) NO and (B) ROS was measured using the Griess systems and flow cytometry, respectively. For NO measurement, cells were incubated with varied concentrations of 1 (2.5, 5 or 10 μM) or TAK-242 (2.5, 5 or 10 μM) and treated with LPS (1 μg/mL) for 24 h. For ROS determination, cells were incubated with 1 (10 μM), TAK-242 (10 μM) or NAC (10 mM) and treated with LPS (1 μg/mL) for 24 h. Results are representative of three independent experiments. ### p < 0.005 vs. control cells, *** p < 0.005 vs. LPS-induced cells.