Figure 5.

The concentration- and time-dependent inhibitory effects of (A) EVF, (B) SVF-5Ac, and (C) SVF on genotype 2a HCV protein expression in J6/JFH1 RNA-transfected Huh7.5 cells. Their impacts on HCV protein expression were measured by Western blot analysis of the HCV NS3 protein in J6/JFH1 RNA-transfected Huh7.5 cells. EC₅₀ is the concentration required for 50% inhibition of HCV NS3 protein expression. T_1/2 is the time required for 50% inhibition of HCV NS3 protein expression at the concentration of 10 μM. Positions of 55 and 70 kd protein size markers were indicated with lines.