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. 2019 Oct 4;8(10):1617. doi: 10.3390/jcm8101617

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Transepithelial electrical resistance, relative permeability and immunofluorescence staining of S. aureus exoproteins treated HNEC-ALI cultures. (a,b) ½ MIC clindamycin significantly reversed the TEER decrease (a) and the increased HNEC-ALI permeability of FITC-dextrans induced by S. aureus ATCC 51650, CI1 and CI2 (b). (c) Immunofluorescence staining of ZO-1 (green) in HNEC-ALI cultures treated with medium (control) or exoproteins from ATCC 51650, CI1 and CI2 left untreated or treated with ½ MIC clindamycin or ½ MIC azithromyxin. DAPI stains nuclei blue. * p < 0.05, ** p < 0.01. n = 3.

Transepithelial electrical resistance, relative permeability and immunofluorescence staining of S. aureus exoproteins treated HNEC-ALI cultures. (a,b) ½ MIC clindamycin significantly reversed the TEER decrease (a) and the increased HNEC-ALI permeability of FITC-dextrans induced by S. aureus ATCC 51650, CI1 and CI2 (b). (c) Immunofluorescence staining of ZO-1 (green) in HNEC-ALI cultures treated with medium (control) or exoproteins from ATCC 51650, CI1 and CI2 left untreated or treated with ½ MIC clindamycin or ½ MIC azithromyxin. DAPI stains nuclei blue. * p < 0.05, ** p < 0.01. n = 3.