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. 2019 Oct 10;24(20):3648. doi: 10.3390/molecules24203648

Figure 4.

Figure 4

Effect of pH on the yield of DFF of co-immobilized tri-enzymes (■), separately immobilized tri-enzymes (▲), and free tri-enzymes (●). Reaction conditions: 35 mg of co-immobilized tri-enzymes or 105 mg of separately immobilized tri-enzymes (containing 35 mg of immobilized galactose oxidase, 35 mg of immobilized catalase and 35 mg of immobilized horseradish peroxidase) or free tri-enzymes (20 U of galactose oxidase, 8000 U of catalase and 500 U of horseradish peroxidase); 200 mM of HMF; 25 °C; at different pH values ranging from 5.0 to 8.0. The buffers used were citric acid–sodium citrate (pH 5.0–6.6), and Tris-HCl (pH 7.1–8.0), respectively.