Table 2.
Representative covalent and regioselective (C+R+) DNA-protein conjugation methods.
| C+R+ (Covalent/Regioselective Methods) | ||
|---|---|---|
| DNA | Protein | DNA-Protein Conjugate |
| Cu(I)-catalyzed azide-alkyne cycloaddition [34] | ||
|
|
|
| Cu(I)-free azide-alkyne cycloaddition [35] | ||
|
|
|
| Staudinger ligation [36] | ||
|
|
|
| SNAP tag [37] | ||
|
|
|
| Halo-tag [38] | ||
|
|
|
| Expressed protein ligation [39,40] | ||
|
|
|
| Microbial transglutaminase [41] | ||
|
|
|
| Sortase A [42,43] | ||
|
|
|
| Terminal deoxynucleotidyl transferase [44] | ||
|
|
|
| DNA-templated conjugation [45] | ||
|
|
|