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. 2019 Oct 22;11(10):609. doi: 10.3390/toxins11100609

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Biochemical properties of recombinant CotA laccase from Bacillus subtilis (BsCotA). (A) Purification of the recombinant BsCotA. Lane 1, Marker; 2, BsCotA. (B) UV–visible spectrum of BsCotA. (C) Effect of pH on BsCotA activity. The substrates used were 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,6-dimethoxy phenol (2,6-DMP), and syringaldazine (SGZ). (D) Effect of temperature on BsCotA activity. (E) pH Stability. (F) Thermostability. For (D–F), the activity was assayed using ABTS as the substrate. Each assay was carried out with three independent biological replicates.