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. 2019 Oct 15;11(10):949. doi: 10.3390/v11100949

Table 3.

Detection of JEV in pig herds with reproductive failure and quantification of reproductive disorders.

Lab Method Material Result Differential Diagnosis Intention Reproductive Failure Country Reference
RT-PCR Aborted fetuses Detection No No quantitative data China [68]
RT-PCR Sample of 37 CSF of aborted piglets 5/37 No No quantitative data China [69]
ICISM, virus isolation, RT-PCR Sample of 108 brain tissues of stillborn piglets 20/108 No No quantitative data China [70]
RT-PCR Sample of 3 brain samples of stillborn piglets 3/3 CSFV, PRRSV, PRV, PPV not detected 30 sows with RF/200 sows (15%) China [64]
RT-PCR 31 brain samples of stillborn piglets (all stillborn piglets of the farm) 7/31 No 10 sows with RF/28 sows (36%), 2–5 sb/sow, 31 sb in total India [65]
RT-PCR Sample of 8 brain samples of stillborn piglets 8/8 CSFV, PRRSV, PRV, PPV not detected 37 sows with RF/128 sows (29%) China [66]
HIA on body fluids and virus isolation (unspecified method) Aborted fetuses Isolation on “some” fetuses No 50 sows with RF/320 sows (3 farms) (16%) Japan [50]

RT-PCR—reverse transcription-polymerase chain reaction; ICISM—intracerebral inoculation of suckling mice; HIA—hemagglutination inhibition assay; CSF—cerebrospinal fluid; CSFV—classical swine fever virus; PRRSV—porcine respiratory syndrome virus; PRV—pseudorabies virus; PPV—porcine parvovirus; sb—stillborn; RF—reproductive failure (abortion or at least one stillborn piglet in the litter).