Figure 4.

Cells treated with DON showed significantly reduced levels of protein synthesis compared to controls. De novo protein synthesis was detected in populations of MDBK cells exposed to DON/DMSO or DMSO (control) by production of a green signal, visible by immunohistology. The intensity of this signal in cytoplasm (A) and nuclei (B) was quantified, demonstrating that treatment with DON/DMSO reduced this signal; a representative analysis from single images is shown. Exposure to DON/DMSO significantly reduced de novo protein synthesis when compared to control populations of cells (C); data are shown from five replicate experiments and are expressed as mean + standard deviation (SD). Representative 20× magnified images from cells treated with DMSO (D) and DON/DMSO (E) are shown; green represents de novo protein synthesis and blue is a DAPI nuclear stain. **** indicates p < 0.0001.