Table 2.
Extract/ Fraction |
IC50 (mg/mL) | Activity Equivalent to Standard (mg TE/g) | |||
---|---|---|---|---|---|
DPPH | ABTS | FICA | FRAP | ORAC | |
Crude methanol extract | 3.19 ± 0.02 a | 0.56 ± 0.01 a | 9.23 ± 0.19 a | 0.26 ± 0.03 a | 1.61 ± 0.19 a |
Hexane fraction | 6.22 ± 0.01 b | 0.54 ± 0.01 b | 2.58 ± 0.03 bc | 1.93 ± 0.35 b | 0.57 ± 0.07 bc |
Chloroform fraction | 3.29 ± 0.02 c | 0.44 ± 0.01 c | 2.43 ± 0.01 c | 2.19 ± 0.23 b | 0.77 ± 0.05 b |
Ethyl acetate fraction | 3.17 ± 0.04 a | 0.41 ± 0.02 d | 2.22 ± 0.01 bc | 8.51 ± 0.09 c | 1.44 ± 0.29 a |
Aqueous fraction | 3.91 ± 0.03 d | 0.45 ± 0.03 c | 2.71 ± 0.02 b | 1.23 ± 0.21 d | 0.44 ± 0.09 c |
Trolox (standard) | 0.011 ± 0.00 e | 0.008 ± 0.00 e | - | - | - |
EDTA (standard) | - | - | 0.019 ± 00 d | - | - |
Results are expressed as mean ± SD; n = 4. DPPH (1,1-diphenyl-2-picrylhydrazine); ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)); FICA (ferrous iron chelating activity); FRAP (ferric reducing antioxidant powder); ORAC (oxygen radical absorbance capacity); EDTA (ethylenediaminetetraacetic acid); TE (Trolox equivalent). Mean values in a column superscripted by different letters (a–e) are significantly different at p < 0.05.