Table 1.
Data obtained from culicinae pools captured in different climate periods at High Pantanal, Central-Western Brazil and subjected to high throughput sequencing.
| Period | Plots | Pools | Species (n) | Viral Hit | Virus | Genome | Size (nt) | Identity (%) | E-value | Reads | Viral Isolation | Genbank |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Rainy | A2; A3 | M05, M06 | Psorophora albigenu (100; 106) | Rhabdoviridae (Dielmovirus) | Lobeira virus | ssRNA- | 11,275 | 100 | 0 | 24,452,482 | Nt | MK780203 |
| Transitional | A1 | M14 | Psorophora albigenu (110) | Iflaviridae (Iflavirus) | Pirizal iflavirus | ssRNA+ | 9367 | 33 | 3e-175 | 10,209,066 | +p1, 300 pb | MN186291 |
| A1 | M22 | Aedes (Ae) aegypti, Ae. Fluviatillis, Ae. crinifer (5) | Flaviviridae (Flavivirus) | Chacororé flavivirus | ssRNA+ | 461 | 55 | 8e-37 | 13,768,232 | nt | MK780202 | |
| Dry | D1 | M25* | Aedes scapularis (7) | Phenuiviridae (Phlebovirus) | Furrundu phlebovirus | ssRNA- | L 7706 | 62 | 0 | 11,579,630 | **500pb | MN186294 |
| M 934 | 30 | 2e-88 | MN186295 | |||||||||
| S 1402 | 40 | 7e-58 | MN186296 | |||||||||
| A3 | M31 | Sabethes gymonothorax (3) | Flaviviridae (Flavivirus) | Sabethes flavivirus | ssRNA- | 1623 | 94 | 6e-95 | 17,431,974 | Nt | MK780204 | |
| Chuviridae (Mivirus) | Uru chuvirus | ssRNA- | 864 | 69 | 3e-150 | MK780200 | ||||||
| Reoviridae (Orbivirus) | Rasqueado orbivirus | dsRNA | 2854 | 38 | 0 | MK780201 | ||||||
| Phenuiviridae (Phlebovirus) | Pixé phlebovirus | ssRNA- | 444 | 39 | 5e-21 | MN186293 | ||||||
| Partitiviridae (Unclassified) | Araticum virus | dsRNA | 1299 | 99 | 0 | MK780207 | ||||||
| A2; A3 | M33 | Coquillettidia albicosta, Coq. shannoni (14) | Rhabdoviridae (Vesiculovirus) | Guampa vesiculovirus | ssRNA- | 2387 | 67 | 2e-75 | 12,498,218 | +p1, 300 pb | MN225577 | |
| A1; A2 | M35* | Aedes scapularis (11) | Phenuiviridae (Phlebovirus) | Furrundu phlebovirus | ssRNA- | L 7706 | 62 | 0 | 10,240,634 | **500pb | MN186294 | |
| M 934 | 30 | 2e-88 | MN186295 | |||||||||
| S 1402 | 40 | 7e-58 | MN186296 | |||||||||
| A1; D1 | M37 | Psorophora. albigenu (100) | Totiviridae (Artivirus) | Murici virus | dsRNA | 907 | 99 | 0 | 13,477,440 | Nt | MK780210 | |
| Rhabdoviridae (Dielmovirus) | Lobeira virus | ssRNA | 450 | 99 | 1e-96 | MK780209 | ||||||
| Partitiviridae (Unclassified) | Araticum virus | dsRNA | 1439 | 99 | 0 | MK780208 | ||||||
| D1 | M38 | Psorophora albigenu (117) | Circoviridae (Krikovirus) | Bororo circovirus | ssDNA | 768 | 78 | 6e-103 | 10,520,776 | **760 pb | MN186292 |
* Same virus. N: number; nt: nucleotide; -: negative; +: positive; ss: single-stranded; ds: double-stranded; L: Large, M: medium, S: small segment. ** Detected directly from the macerated pool.