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. 2019 Oct 15;8(10):1686. doi: 10.3390/jcm8101686

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(a) The scheme of estimation of penetration profile in spheroid cryosections. (b–d) Penetration profiles of (b) mTHPC, (c) Ce6 (4.5 µM) and (d) ICG (40 µM) in FaDu (black), FaDu:MeWo 5:2 (red) and FaDu:MeWo 5:5 (blue) spheroids at 5-day post-seeding after incubation for 24 h. ^†: statistically significant (p < 0.05) difference in the range of 50–110 µm between F5 and F5M2/F5M5 treated with mTHPC, ^‡: statistically significant (p < 0.05) difference in the range of 20–240 µm between F5 and F5M2/F5M5 treated with Ce6.