Icariin administration for 30 days increased the stand-up times (A) and movement distance (B) in the open field test and decreased immobility time (C) in the forced swimming test in perimenopausal depression rats. Sham group (SG), model group (MG), estradiol valerate group (EVG, 0.167 mg/kg), high-dose icariin group (IG-HD, 50 mg/kg), medium-dose icariin group (IG-MD, 25 mg/kg), low-dose icariin group (IG-LD, 12.5 mg/kg). Values are expressed as mean ± S.D, n = 10. ## p < 0.01 versus the SG. ** p < 0.01 versus the MG. * p < 0.05 versus the MG. (Statistical analyses were performed using ANOVA, and the criterion for statistical significance was set to p < 0.05).