Icariin administration for 30 days increased the expression of bax (Figure 7A) and B-cell lymphoma 2 (Bcl-2) (Figure 7B) in perimenopausal depression rats. Sham group (SG), model group (MG), estradiol valerate group (EVG, 0.167 mg/kg), high-dose icariin group (IG-HD, 50 mg/kg), medium-dose icariin group (IG-MD, 25 mg/kg), and low-dose icariin group (IG-LD, 12.5 mg/kg). Values are expressed as mean ± S.D, n = 10. ## p < 0.01 versus the SG. ** p < 0.01 versus the MG. * p < 0.05 versus the MG. Representative photomicrographs of SG (C and I), MG (D and J), EVG (E and K), IG-HD (F and L), IG-MD (G and M), and IG-LD (H and N) (Immunofluorescence ×400).(Statistical analyses were performed using ANOVA, and the criterion for statistical significance was set to p < 0.05).