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. 2019 Nov 6;39(45):8877–8884. doi: 10.1523/JNEUROSCI.0973-19.2019

Figure 3.

Figure 3.

Decreasing F-actin turnover in the MB during pre-exposure suppresses EDAP. A, Brain schematic highlighting the MB. B, Experimental design with RU486 feeding before ethanol exposure to activate the MB-GeneSwitch Gal4 driver. C, Decreasing F-actin turnover via three different transgenes did not alter naive alcohol avoidance (one-way ANOVA, F(3,56) = 2.6, p = 0.06, ns, not significant, n = 12; 24 for GFP control). D, Decreasing F-actin turnover with transgene activation before ethanol pre-exposure prevented EDAP (one-way ANOVA, F(3,73) = 15.0, p < 0.0001; Dunnett's multiple post hoc comparison ***p ≤ 0.0008, n = 12–18; 29 for GFP control). E–G, Activating MB-GeneSwitch after alcohol pre-exposure had no effect on naive avoidance (one-way ANOVA, F(3,32) = 0.7, p = 0.55, n = 6; 18 for GFP control; F), or on EDAP (one-way ANOVA, F(3,32) = 1.4, p = 0.27, n = 6; 17 for GFP control; G).