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. Author manuscript; available in PMC: 2020 Apr 22.
Published in final edited form as: ACS Nano. 2019 Sep 30;13(10):12148–12161. doi: 10.1021/acsnano.9b06691

Figure 7.

Figure 7.

Tumor microenvironment analysis, ELISPOT (enzyme-linked immunospot) assay, and H&E staining of major organs. C57BL/6 mice were randomly divided into the following six treatment groups: (1) PBS control; (2) soluble vaccine (CpG, Ce6, and Adpgk peptide); (3) soluble vaccine with laser irradiation; (4) bMSN(Ce6) with laser irradiation; (5) bMSN vaccine (bMSN(CpG/Ce6)-Adpgk); and (6) bMSN vaccine with laser irradiation. The laser irradiation (660 nm, 50 mW/cm2 for 15 min) was conducted to the tumor area 24 h after each injection in the laser irradiation group. (a) Seven days postimmunization, the IFN-γ ELISPOT assay was performed by ex vivo restimulation of splenocytes with Adpgk peptides (10 μg/mL). In parallel, tumor tissues were analyzed for the frequencies of CD8α+ T-cells (b), Adpgk-specific CD8α+ T-cells (c), activated CD11c+CD86+ DCs (d), and NK cells (e) using flow cytometry. (f) Hematoxylin–eosin (H&E) staining images of major mice organs in the PBS group and the bMSN(CpG/Ce6)-Adpgk group on day 30 after immunization.