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. Author manuscript; available in PMC: 2020 Nov 4.
Published in final edited form as: Curr Biol. 2019 Oct 24;29(21):3635–3646.e5. doi: 10.1016/j.cub.2019.08.079

Figure 7. The EB fragmentation circuit has high interconnectivity but limited output.

Figure 7.

5 confocal sections at different levels of the same brain from anterior to posterior are shown for trans-Tango targeted brain regions with VT-GAL4 (A-E) and 5HT7-GAL4 (F-J). Expression of the GAL4 marked with GFP is shown in green, trans-Tango expressing mtdTomato in magenta and white indicates GAL4+ neurons making synapses on themselves. VT-GAL4+ neurons target locally in the central complex: ca. 2–3 GAL4+ neurons and a population of ca. 60 other EB neurons in the region of 5HT7-GAL4. Fan-shaped body (FB) and protocerebral bridge (PB) also receive synapses. 5HT7-GAL4+ neurons target themselves within the EB, and output to mushroom body (MB) α’β’ lobes, FB and PB. (K) Proposed model for the circuit controlling sleep structure. See also Figure S7D, Videos S4-5.