KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-GFP | Roche Applied Science | Cat#11814460001 RRID:AB_390913 |
| Rabbit polyclonal anti-GFP | Invitrogen | Cat# A-11122 RRID: AB_221569 |
| Rabbit anti-serotonin | Sigma | Cat# S5545 RRID: AB_477522 |
| Rabbit polyclonal anti-Ds-Red | Clontech | Cat# 632496 RRID: AB_10013483 |
| Chicken anti-GFP | Abcam | Cat# 13970 RRID: AB_300798 |
| Rabbit anti-hCD4 | Novus | Cat# NBP1–86143 RRID: 11037580 |
| Alexa Fluor 488 (goat anti-mouse) | Invitrogen | Cat# A-11001 RRID: AB_2534069 |
| Alexa Fluor 488 (goat anti-rabbit) | Invitrogen | Cat# A-11008 RRID: AB_143165 |
| Alexa Fluor 488 (goat anti-chicken) | Invitrogen | Cat# A-11039 RRID: AB_142924 |
| Alexa Fluor 633 (goat anti-mouse) | Invitrogen | Cat# A-21052 RRID:AB_2535719 |
| Alexa Fluor 635 (goat anti-rabbit) | Invitrogen | Cat# A-31576 RRID: AB_2536186 |
| Vectashield | Vector Laboratories, Inc. | Cat# H-1000 RRID: AB_2336789 |
| Bacterial and Virus Strains | ||
| pBPnlsLexA::p65Uw | [55] | Addgene Cat#26230 |
| NEB Stable Competent E. coli (High Effiency) | NEB | Cat# C3040H |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Tetrodotoxin (TTX) | Tocris Bioscience | Cat# 1078 |
| Adenosine 5’-triphosphate magnesium salt (ATP) | Sigma | Cat# A9187 |
| Serotonin hydrochloride (5-HT) | Sigma | Cat# H9523 |
| Gaboxadol Hydrochloride (THIP) | Sigma | Cat# T101 |
| SB258719 hydrochloride | Tocris Bioscience | Cat# 2726 |
| D-Luciferin Firefly, potassium salt | BIOSYNTH AG | Cat# L-8220 |
| Critical Commercial Assays | ||
| SuperScript III First-Stand Synthesis System | Invitrogen | Cat# 18080 |
| QuantiFast SYBR Green PCR kit | Qiagen | Cat# 204054 |
| Experimental Models: Organisms/Strains | ||
| Drosophila: UAS-dTrpA1 | [18] | RRID: BDSC_26263 |
| Drosophila: UAS-Kir2.1 | [56] | FlyBase: FBtp0039831 |
| Drosophila: UAS-Chrimson-tdTomato | [57] | |
| Drosophila: UAS-mCD8::GFP | [58] | RRID: BDSC_5136 |
| Drosophila: UAS-mCD8::RFP,LexAop2-mCD8::GFP | [55] | RRID: BDSC_32229 |
| Drosophila: Trh-GAL4 (II) | Bloomington Drosophila Stock Center | RRID: BDSC_38388 |
| Drosophila: Trh-GAL4 (III) | Bloomington Drosophila Stock Center | RRID: BDSC_38389 |
| Drosophila: PBac{WH}5-HT7f05214 | Bloomington Drosophila Stock Center | RRID: BDSC_18848 |
| Drosophila: Mi{MIC}5-HT7MI00215 | Bloomington Drosophila Stock Center | RRID: BDSC_30667 |
| Drosophila: Mi{ET1}5-HT7MB01344 | Bloomington Drosophila Stock Center | RRID: BDSC_23066 |
| Drosophila: Mi{ET1}5-HT7MB04445 CG31008MB04445 | Bloomington Drosophila Stock Center | RRID: BDSC_24705 |
| Drosophila: Mi{MIC}5-HT7MI08096 | Bloomington Drosophila Stock Center | RRID: BDSC_44745 |
| Drosophila: UAS-GCaMP6f | Bloomington Drosophila Stock Center | RRID: BDSC_42747 |
| Drosophila: UAS-nSyb:spGFP1–10, LexAop-CD4:spGFP11 | [45] | RRID: BDSC_64314 |
| Drosophila: LexAop-nSyb:spGFP1–10, UAS-CD4:spGFP11 | Bloomington Drosophila Stock Center | RRID: BDSC_64315 |
| Drosophila: LexAop-P2X2 | [46] | RRID: BDSC_76030 |
| Drosophila: VT038828-GAL4 | Vienna Drosophila Resource Center (VDRC) | RRID: BDSC_v201975 |
| Drosophila: UAS-Epac1-camps(55A) | [59] | Dr. Orie Shafer |
| Drosophila: 5-HT7Dro-GAL4 | [37] | Dr. Charles Nichols |
| Drosophila: UAS-myrGFP,QUAS-mtdTomato-3xHA;trans-Tango | [47] | RRID: BDSC_77124 |
| Drosophila: Tric-LUC | [23] | Dr. Michael Rosbash |
| Drosophila: R23E10-GAL4 | [60] | RRID:BDSC_49032 |
| Oligonucleotides | ||
| RT forward primer for 5-HT7: GCATGGTGCGGAAATTGAGG | This paper | N/A |
| RT reverse primer for 5-HT7: CCACGGATATGGCACACAGA | This paper | N/A |
| RT forward primer for RPL32: GGACAGTATCTGATGCCCAAC | This paper | N/A |
| RT reverse primer for RPL32: ATCTCGCCGCAGTAAAGGC | This paper | N/A |
| Amplification for VT038828 promoter forward primer: AGTTTTTCCCATTTCCCATCAACAAAA | http://stockcenter.vdrc.at/control/product/~VIEW_INDEX=0/~VIEW_SIZE=100/~product_id=201975 | N/A |
| Amplification for VT038828 promoter reverse primer: CCGGAGGACCCCAGGACTATGTCTAC | http://stockcenter.vdrc.at/control/product/~VIEW_INDEX=0/~VIEW_SIZE=100/~product_id=201975 | N/A |
| Sequencing forward primer for VT038828-LexA: CAGGGTTATTGTCTCATGAGCGGATAC | This paper | N/A |
| Sequencing reverse primer for VT038828-LexA: ACGTCTGCTCGGCTCGAACATTCATTC | This paper | N/A |
| Software and Algorithms | ||
| Fiji | http://fiji.sc | RRID: SCR_002285 |
| GraphPad Prism 7 | GraphPad Software | RRID: SCR_002798 |
| MATLAB R2012b | MathWorks | RRID: SCR_001622 |
| Leica TCS SP5 confocal microscope | Leica | RRID: SCR_002140 |