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. Author manuscript; available in PMC: 2020 Nov 15.
Published in final edited form as: J Immunol. 2019 Oct 2;203(10):2621–2629. doi: 10.4049/jimmunol.1900784

Figure 2. Anti-CD3-induced oral tolerance is abrogated in the absence of cDC, but not pDC.

Figure 2.

(A) Scheme for testing tolerance induction by oral anti-CD3 in the EAE model with or without depletion of conventional dendritic cells (cDCs) or plasmocytoid DCs (pDCs). (B, C) Depletion of plasmocytoid (pDC) and conventional dendritic cells (cDC) in the mLN (B) and spleen (C) from BDCA2-DTR and zDC-DTR mice 48 h after 20 ng of diphtheria toxin (DT) injection. (D, E) EAE clinical score of of DT-treated BDCA2-DTR mice (D), zDC-DTR mice (E) or respective littermate controls with or without previous feeding with anti-CD3; n=7-10 mice/group. Data are shown as mean ± SEM and are representative of two independent experiments. Two-way ANOVA was used. * p<0.05, ** p<0.01. NS= not significant.