Table 3.
PCR Primers for Fluorescence Real-Time Quantitative PCR.
| Gene | Forward (5′-3′) | Reverse (5′-3′) |
|---|---|---|
| cviI | GAAACCGTCCTCGCATAAGG | ACAAGGTGGACTGGTACTGG |
| cviR | CCCAGCAATATGCCGCTATC | CATTGAGCTTGCGGATCACA |
| vioA | AAGAGCATGGCAAGGAATC | CTGGTTGGCGTCGTTATC |
| vioB | CTGGGCGTAATTGGGAATGG | CAAATACCTGGCCCATGTCG |
| vioC | GAACAAGTACGCCAACCT | GGAAGAAAGTCTGCTGGAA |
| vioD | GCCGCAACAAGTACATCT | GAAGGTGCTCATCGTGTC |
| vioE | ATAGGCCACCTTCTGCTTCC | GGCTACTGCTGGTTCGACTA |
| hmsH | CGCCGTATGTCTTCAGTT | CGCAGCCTATCGTAGATG |
| hmsF | GGCTGCTGATTCTCTGTTA | GTATAGACGCTGCGGTAG |
| hmsN | AAACCACACGCACCAGAAC | TGCATGAGCATGAAGACGAC |
| lasA | AGCCAGCCTTACGATTCCAT | GAGGAATAGCCGTTGTCGTG |
| lasB | AGAACGCCTTGTTGTACACG | GCAAGAACGACTTCCTGGTC |
| pilE3 | TACGCTGGTCGAGTTGATGA | GCGAATAGCACTGCTCCATC |
|
rpoD
(actin) |
TCGGACATCAGCAAGGTT | GTGAAGGACAGCCAACAG |