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. 2019 Oct 16;24(20):3726. doi: 10.3390/molecules24203726

Figure 5.

Figure 5

Insulin-stimulated glucose uptake by differentiated primary rat preadipocytes cultured with (OTA+) or without the addition of ochratoxin A (OTA-) in cell culture medium and with the addition of quercetin (QCT) at various concentrations (A). 2-deoxyglucose uptake was determined using a commercially available kit. The gene expression of glucose transporter 4 (Glut4; B) and insulin receptor substrate 1 (Irs1; C) was determined by real-time PCR. Data were normalized to the gene expression of 40S ribosomal protein S29 (Rps29), the expression of which was not altered by the treatments. The results, presented as mean ± mean error (SEM), were analyzed by two-way ANOVA with factors QCT and OTA, followed by Bonferroni post-hoc test. The level of statistical significance considered for factor OTA was * p < 0.05; ** p < 0.01; *** p < 0.001, and for factor QCT # p < 0.05; ## p < 0.01; ### p < 0.001.