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. 2019 Sep 27;8(10):1556. doi: 10.3390/jcm8101556

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Autophagy levels in PZA- and INH-treated granulomas. Autophagy was measured with an anti-LC3B antibody, conjugated with PE-Cy5. The nuclei of cells were stained with DAPI. The mean fluorescence of LC3B was corrected with the mean DAPI fluorescence. (A) LC3B mean fluorescence from non-vaccinated subjects with L-GSH treatment, alongside LC3B fluorescent images; (B) LC3B mean fluorescence from BCG-vaccinated subjects with L-GSH treatment, alongside LC3B fluorescent images; (C) LC3B mean fluorescence with PZA, 1/10 PZA, and 1/100 PZA treatment in the presence or absence of L-GSH in non-vaccinated subjects; (D) LC3B mean fluorescence with PZA, 1/10 PZA, and 1/100 PZA treatment in the presence or absence of L-GSH in BCG-vaccinated subjects; (E) LC3B mean fluorescence in non-vaccinated and BCG-vaccinated groups with sham treatment and L-GSH treatment; (F) LC3B mean fluorescence in non-vaccinated and BCG-vaccinated groups with PZA treatment. Data represents ± SE from experiments performed from 14 different subjects. An unpaired t-test with Welch corrections was used in Figure (A). Analysis of Figures B–F utilized a one-way ANOVA with Tukey test. The p value style consisted of 0.1234 as not significant, 0.0332 with one asterisk (*), 0.0021 with two asterisks (**), 0.0002 with three asterisks (***), and less than 0.0001 with four asterisks (****). A hash mark (#) indicates categories compared to the control, and an asterisk indicates categories compared to the previous category directly before it. (G) LC3B mean fluorescence with INH, 1/10 INH, and 1/100 INH treatment in the presence or absence of L-GSH in non-vaccinated subjects; (H) LC3B mean fluorescence with INH, 1/10 INH, and 1/100 INH treatment in the presence or absence of L-GSH in BCG-vaccinated subjects; (I) LC3B mean fluorescence in non-vaccinated and BCG-vaccinated groups with INH treatment. Data represents ± SE from experiments performed from 14 different subjects. Analysis of figures utilized a one-way ANOVA with Tukey test. The p value style consisted of 0.1234 as not significant, 0.0332 with one asterisk (*), 0.0021 with two asterisks (**), 0.0002 with three asterisks (***), and less than 0.0001 with four asterisks (****). A hash mark (#) indicates categories compared to the control, and an asterisk indicates categories compared to the previous category directly before it.

Autophagy levels in PZA- and INH-treated granulomas. Autophagy was measured with an anti-LC3B antibody, conjugated with PE-Cy5. The nuclei of cells were stained with DAPI. The mean fluorescence of LC3B was corrected with the mean DAPI fluorescence. (A) LC3B mean fluorescence from non-vaccinated subjects with L-GSH treatment, alongside LC3B fluorescent images; (B) LC3B mean fluorescence from BCG-vaccinated subjects with L-GSH treatment, alongside LC3B fluorescent images; (C) LC3B mean fluorescence with PZA, 1/10 PZA, and 1/100 PZA treatment in the presence or absence of L-GSH in non-vaccinated subjects; (D) LC3B mean fluorescence with PZA, 1/10 PZA, and 1/100 PZA treatment in the presence or absence of L-GSH in BCG-vaccinated subjects; (E) LC3B mean fluorescence in non-vaccinated and BCG-vaccinated groups with sham treatment and L-GSH treatment; (F) LC3B mean fluorescence in non-vaccinated and BCG-vaccinated groups with PZA treatment. Data represents ± SE from experiments performed from 14 different subjects. An unpaired t-test with Welch corrections was used in Figure (A). Analysis of Figures B–F utilized a one-way ANOVA with Tukey test. The p value style consisted of 0.1234 as not significant, 0.0332 with one asterisk (*), 0.0021 with two asterisks (**), 0.0002 with three asterisks (***), and less than 0.0001 with four asterisks (****). A hash mark (#) indicates categories compared to the control, and an asterisk indicates categories compared to the previous category directly before it. (G) LC3B mean fluorescence with INH, 1/10 INH, and 1/100 INH treatment in the presence or absence of L-GSH in non-vaccinated subjects; (H) LC3B mean fluorescence with INH, 1/10 INH, and 1/100 INH treatment in the presence or absence of L-GSH in BCG-vaccinated subjects; (I) LC3B mean fluorescence in non-vaccinated and BCG-vaccinated groups with INH treatment. Data represents ± SE from experiments performed from 14 different subjects. Analysis of figures utilized a one-way ANOVA with Tukey test. The p value style consisted of 0.1234 as not significant, 0.0332 with one asterisk (*), 0.0021 with two asterisks (**), 0.0002 with three asterisks (***), and less than 0.0001 with four asterisks (****). A hash mark (#) indicates categories compared to the control, and an asterisk indicates categories compared to the previous category directly before it.