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. 2019 Oct 13;24(20):3682. doi: 10.3390/molecules24203682

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Time-course effect of the IL-1β and IL-17A combination on the expression of the genes associated with the cartilaginous matrix-degrading enzymes in porcine pellet culture. The pellets were cultured for up to 28 days under conditions in which they were stimulated by the combined cytokines (0.25 ng/mL of IL-1β in combination with 0.5 ng/mL of IL-17A). The untreated pellets were left as a control (Ctrl). The mRNA expressions of the pellets were analyzed by real-time qRT-PCR at the indicated times. Data are expressed as mean ± SD of two independent experiments. * = p < 0.05 and ** = p < 0.001.