Figure 4.
ISL blocked intraperitoneal xenograft development and prolonged the survival of ovary-tumor-bearing mice. (A) Flowchart of therapy scheme. (B) Luciferase-containing SKOV3 cells (5 × 106 cells/mouse) were injected intraperitoneally into nude mice for 3 weeks, followed by administration of 12.5 or 25 mg/kg of ISL. Tumors were imaged in the Xenogen system. (C) Kaplan–Meier analysis of animal endpoint survival following treatment with ISL. (D) Body weight was measured every week. (E) The mRNA expression of E-cad, Vim, and ZEB1 was detected in tumor tissues. Gene expression is presented as fold changes and normalized to β-actin. (F) Representative pictures of immunohistochemical staining of E-cadherin, Vim, and ZEB1 in tumor tissues. Scale bars, 20 μm. Data are presented as mean ± SD, n = 6. Student t-test was used for statistical tests. * p < 0.05, ** p < 0.01 compared with the vehicle group.