Table 1.

Sequences of primers designed in our study.

Primer	Sequence (5'-3')	Restriction Enzyme Site
MF	ACAGCTAGCCGGTCCGATGACCGAGATCTACGAT	NheI+RsrII
MR	GCATGCGCGGCCGCTTACAGGATCTTGAACAG	SphI+NotI
FF	ACAGCTAGCCGGTCCGATGACACGGGTCGCAACC	NheI+Rsr II
FR	GCATGCGCGGCCGCCTACAGTGATCTCACGTA	SphI+NotI
HF	ACAGCTAGCCGGTCCGATGTCCGCACAAAGGGAA	NheI+RsrII
HR	GGTACCGCGGCCGCTCAGACTGGATTACATGT	KpnI+NotI
M13F	GTTTTCCCAGTCACGAC
M13R	CAGGAAACAGCTATGAC
P10F	GGGGTATCGACAGAGTGC
P10R	CGGACCTTTAATTCAACCC

The sequences of restriction enzyme sites are underlined.