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. 2019 Nov 6;12:261. doi: 10.1186/s13068-019-1601-x

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Heterologous biosynthesis of icariside D2 in E. coli BL21 (DE3) from tyrosol. a HPLC map of fermentation broth. b Production of icariside D2 by heterologous expression of RcUGT1, RrUGT3, and RsUGT72B14. c Time profile of icariside D2 fermentation from tyrosol by strain BMD2 expressing RrUGT3