Fig. 2.

Function of CKS1 in HCT116 and SW620 cells. pCDNA3.1-CKS1-RFP plasmids were transfected to HCT116 and SW620 cells to study the function of CKS1 in colon cancer cells, pCDNA3.1-RFP plasmids transfected cells were used as controls; a CCK-8 assay was used to measure the proliferation ratio of HCT116 cells post plasmid was transfected for 12 h, 24 h and 48 h, respectively; b CCK-8 assay was used to measure the proliferation ratio of SW620 cells post plasmid was transfected for 12 h, 24 h and 48 h, respectively; c the colony formation ability of HCT116 cells was measured and statistically analyzed using a colony formation assay following 10 days of culture; d the colony formation ability of SW620 cells was measured and statistically analyzed using a colony formation assay following 14 days of culture. *Indicates p < 0.05 for three independent experiments analyzed by Student’s t test