Fig. 3.

BaCl₂ increases calpain activity. Representative Western blots (top) and mean densitometric data (bottom) for αII-spectrin from EDL muscles treated with standard PSS (Control) or 1.2% BaCl₂ in standard PSS for 1 h. The αII-spectrin band at 240 kDa and its cleavage product at 150 kDa were both normalized to total protein reflected by the 40 kDa band, which was not different between samples. Summary data are means ± SEM (n = 6 muscles). ^#P ≤ 0.05 vs. control