Fig. 5.

Concentration of MEP, IPP/DMAPP, and ME-CDP (a), and MEcPP and DXP (b) in response to changes in dxs expression ($\overline{\mathit{x}}$± SD, n = 3). The graphs for MEP, ME-CDP and DXP are fitted with a linear fit through zero, while IPP/DMAPP is fitted with a power law (R² > 0.95). The intermediate concentrations were measured in the dxs RBS library expressing ispS and idi from pCOLA::IspS-idi. The cultures were induced at OD₆₀₀ = 0.1 and the intermediates were quantified at OD₆₀₀ ≈ 0.5. The concentration of MEcPP in response to dxs expression was fitted to two piecewise linear segments. The graph is divided at the wild-type expression level, with overexpression yielding a slope more than 14-fold steeper than under expression. The metabolite concentration control coefficient of Dxs over MEcPP for the overexpression of dxs is 2.6. Other concentration-control coefficients are given in Additional file 1: Table S9 and were calculated according to Additional file 1: Fig. S8, the double logarithmic plot of the data in this figure