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. 2019 Nov 6;12:523. doi: 10.1186/s13071-019-3781-4

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Nucleotide sequences of the 18S rRNA amplicon and the primer location. Sequence alignment of the nucleotide sequence of the Babesia spp. amplicon used in the HRM analysis. The underlined sequences indicate the position of the primer pair used for the real-time PCR assay