Table 1.

Tm values of average melting curve peak for each Babesia spp.

Species	Tm value range (mean Tm ± SD) (°C)
	DNAa	Variable amount of DNAb
B. bovis	84.15 ± 0.04	84.16 ± 0.19
B. bigemina	80.02 ± 0.02	80.04 ± 0.12
B. major	80.54 ± 0.03	80.62 ± 0.15
B. ovata	81.02 ± 0.05	81.24 ± 0.25
Theileria spp.	79.20 ± 0.19	79.24 ± 0.24

^aThe Tm values obtained from HRM using 20 ng of genomic DNA in 3 repeat reactions in 3 independent experiments

^bThe Tm values obtained from HRM using a variable amount of genomic DNA ranging from 5 to 50 ng in 3 repeat reactions in 3 independent experiments