Table 4.

Oligonucleotide primer sequences for amplification of target equine genes

Gene	Oligo	Sequence (5′-3′)	PCR Product size (bp)	Annealing temperature (°C)	Sequence accession number(s)	Reference(s)
GAPDH	Forward	GGTGAAGGTCGGAGTAAACG	106	64	AF157626; AF083897	Beekman, 2011
	Reverse	AATGAAGGGGTCATTGATGG
HRPT	Forward	AATTATGGACAGGACTGAACGG	121	62	AY372182	Beekman, 2011
	Reverse	ATAATCCAGCAGGTCAGCAAAG
SDHA	Forward	GAGGAATGGTCTGGAATACTG	91	62	DQ402987	Beekman, 2011
	Reverse	GCCTCTGCTCCATAAATCG
RPL-32	Forward	GGGAGCAATAAGAAAACGAAGC	138	62	CX594263	Beekman, 2011
	Reverse	CTTGGAGGAGACATTGTGAGC
IL-1β	Forward	ACCATAAATCCCTGGTGCTG	179	64	D42147; U92481; D42165	Beekman, 2012
	Reverse	CGTCCCACAAGACAGGTACA
IL-4	Forward	TCGTGCATGGAGCTGACTGTA	151	64	L06010; AF035404	Beekman, 2012
	Reverse	GCCCTGCAGATTTCCTTTCC
IL-5	Forward	AAACTGTCCAAGGGGATGCT	169	64	U91947	Beekman, 2012
	Reverse	TCCGTTGTCCACTCAGTGTT
IL-6	Forward	AGCAAGGAGGTACTGGCAGA	173	62	U64794; AF005227; AF041975	Beekman, 2012
	Reverse	CCTTTTCACCCTTGAACTCG
IL-8	Forward	CGCACTCCAAACCTTTCAAT	165	62	AY184956; AF062377	Beekman, 2012
	Reverse	TCAAAAACGCCTGCACAATA
IL-10	Forward	ATCGATTTCTGCCCTGTGAA	174	62	U38200	Beekman, 2012
	Reverse	CGTTCCCTAGGATGCTTCAG
IL-12 p35	Forward	CATGAATGCCAAGCTGTTGA	185	64	Y11130	Beekman, 2012
	Reverse	AGGCATGAAGAAGGATGCAG
IL-17	Forward	TATCGTGAAGGCGGGAATAG	210	62	EU744563	Beekman, 2012
	Reverse	TCCCAGATCACAGAGGGGTA
IL-23	Forward	CTGGCCTGGAGTGCACATC	296	62	AY704416	Hughes, 2011
	Reverse	TTGTAGTCTCAGCATCTCCCTCTTC
IFN-γ	Forward	CTATTACTGCCAGGCCGCGTT	404	64	U04050; D28520	Giguère, 1999
	Reverse	TCCTCTTCCGCTTCCTCAGGTT
Eotaxin-2	Forward	CCTGAGAGCCGAGTGGTAAG	152	64	ENSECAT00000023737	Beekman, 2012
	Reverse	TTCTTGGCAGCCAGATTCTT
TNF-α	Forward	CTTGTGCCTCAGCCTCTTCTCCTTC	1385	64	M64087	Giguère, 1999
	Reverse	CAGCTGGTTGTCTGTCAGCTTC