Fig. 5.

Effect of DNA-PKcs inhibitor Nu7441 on luciferase expression and post-integration gap repair for HIV_wt and HIV_mut. a Relative luciferase expression in 293T and Jurkat cells treated by DMSO or Nu7441 (1 µM for 293T and 5 µM for Jurkat) and transduced by 0.01 MOI of either HIV_wt or HIV_mut. Results are presented as data under Nu7441 treatment normalized to data under DMSO treatment for HIV_wt and HIV_mut separately. Mean values ± SD of n = 7 for 293T and n = 5 for Jurkat cells are presented. b, c Gap repair efficiency estimated for 293T (b) and Jurkat cells (c), data is presented as mean percentage of the total integrated proviral DNA ± SD (n = 5). d Relative gap repair efficiency presented as repair level in Nu7441 treated cells normalized to the level in DMSO-treated cells. Significance was determined by two-tailed Student’s t-test, * = p < 0.05, ** = p < 0.01