Table 1.
The sucrose-producing Synechocystis strains generated and compared in this study
| Strain ID | Genetic background | OE target(s) | Expression plasmid |
|---|---|---|---|
| S01 | Wild-type | CscB | pDF-lac2-cscB-CmR |
| S01:Δflv3 | Δflv3 | CscB | pDF-lac2-cscB-CmR |
| S01:compl | Δflv3 | CscB + Flv3 | pDF-lac2-cscB-flv3-CmR |
| S02 | ΔggpS | CscB + SPS | pDF-lac2-cscB-sps-CmR |
| S02:Δflv3 | Δflv3 + ΔggpS | CscB + SPS | pDF-lac2-cscB-sps-CmR |
The enzymes targeted for inactivation and over-expression are Flv3 (flavodiiron protein 3 from Synechocystis), GGPS (glucosylglycerolphosphate synthase from Synechocystis), CscB (sucrose permease from E. coli), and SPS (sucrose phosphate synthase from Synechocystis)