Table 3.

Outcomes.

Variables	Type of measure	Measurement modality	References
Biomarkers of stress and cardiovascular risk	Skin conductance	Wrist band electrodes – Movisens	7
	Blood flow velocity	Laser speckle contrast imaging	78,79
	Myocardial longitudinal strain	Speckle-tracking echocardiography	85,86
Genetic polymorphisms	Polymorphism of angiotensin converting enzymes	Blood cells	8–11
	Polymorphism of serotonin	Blood cells	12–14
Demographics	Age, gender, qualification, personal work status, ethnicity, life and occupational events	Questionnaire	4
Clinical measurements		Height, weight, blood pressure, heart rate, waist circumference
Body composition	Muscle mass, fat mass, bone structure	Impedance meter	80
		Densitometry X-ray absorption	6
		Peripheral quantitative computed tomography	73–75
		Quantitative ultrasound	81
Psychology and quality of life	Depression	Hamilton Anxiety Rating Scale	62
	Anxiety	Hamilton Anxiety Rating Scale	62
		State–Trait Anxiety Inventory	60,61
	General health	General Health Questionnaire (SF-12)	71
	Stress, fatigue, sleep	Visual analogue scale	4
	Burnout	Maslach Burnout Inventory	59
	Mindfulness	Freiburg Mindfulness Inventory	22,23
	Coping	Brief COPE Questionnaire	63
	Emotions	Emotion Regulation Questionnaire	64
	Perception of work	Karasek’s Job Content Questionnaire	65
	Self-efficacy	Perceived Self-efficacy Scale	66
	Addiction to work	Work Addiction Risk test	67
	Alexithymia	Toronto Alexithymia Scale	68,82,83
	Illness perception	Brief Illness Perception Questionnaire, adapted for stress at work	69,84
	Metacognition	Metacognitions Questionnaire-30	70
Lifestyle	E.g., smoking, alcohol consumption, coffee consumption, food intake	Questionnaires	4
	Physical activity	Recent Physical Activity Questionnaire	72
Alloplastic load	HbA1c, HDLc and LDLc, TG	Ethylenediaminetetraacetic acid tube	6
	Cortisol	Dry tube, serum isolation and deep-freezing	17,85
	DHEAS	Dry tube, serum isolation and deep-freezing	17,85
	Leptin	Dry tube, serum isolation and deep-freezing	6
	BDNF	Dry tube, serum isolation and deep-freezing	20,86,87
	Pro-inflammatory cytokines: IL-1β, IL-6, IL-1, TNFα	Dry tube, serum isolation and deep-freezing	6
	NPY	Dry tube, serum isolation and deep-freezing	19
	Telomere length	Blood; Southern blot or polymerase chain reaction analyses	88
	Heart rate variability	Holter	89
	Skin conductance – electrodermal activity	Wristband electrodes	89

A total of 25 mL of blood will be sampled (i.e. 100 mL in 1 year). HbA1c: haemoglobin A1c; HDLc: high-density lipoprotein cholesterol; LDLc: low-density lipoprotein cholesterol; TG: triglycerides; DHEAS: dehydroepiandrosterone sulphate; BDNF: brain-derived neurotrophic factor; IL: interleukin; TNFα: tumour necrosis factor α; NPY: neuropeptide Y.