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. 2019 Sep 26;8(10):1544. doi: 10.3390/jcm8101544

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Study of TNAP involvement in the mineralization process. (A) TNAP enzymatic activity over time for the two conditions (N = 3). (B) Effects of a TNAP inhibitor on the mineral deposition (N = 3) assessed by the colorimetric method after the solubilization of alizarin red. (C) Representative images of alizarin red staining of wells of one patient. CT: control medium; OM: osteogenic medium; TNAPi: TNAP inhibitor. (D) Gene expression by TLC after treatment with a TNAP inhibitor (N = 3). TNAP: tissue non-specific alkaline phosphatase; ENPP1: ectonucleotide pyrophosphatase/phosphodiesterase; ACAN: aggrecan; COMP: cartilage oligomeric matrix protein; COL10: type X collagen; MMP13: matrix metallopeptidase 13; CT: control; OM: osteogenic medium; TNAPi: TNAP inhibitor; ND: not detected. * = p < 0.05.