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. 2019 Sep 17;20:554–566. doi: 10.1016/j.isci.2019.09.020

Figure 6.

Figure 6

BRCA1 Is Necessary for C286-Mediated Regulation of Pain

(A) Diagram of the experimental design.

(B–D) (B and C) Immunostaining of spinal microglia for BDNF (scale bar, 50 μm) and its quantification. (D) Western blots of spinal cords for BDNF. Student's t test, n = 3 per group, ***p ≤ 0.001.

(E–H) (E and F) Expression and quantification of FZD10 (white arrows show colocalization with Iba1 and black arrows with βIII tubulin) and (G and H) Daxx in spinal neurons and microglia of LV/sc + C286- and LV/BRCA1shRNA + C286-treated rats (insets show its predominant nuclear localization). Scale bars, 100 μm for E and G. Data show Mean FI ± SEM, n = 3 per treatment group, 5 sections per animal. Student's t -test, **p ≤ 0.01, ***p ≤ 0.001.