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. Author manuscript; available in PMC: 2019 Nov 6.
Published in final edited form as: Clin Sci (Lond). 2019 May 9;133(9):1097–1113. doi: 10.1042/CS20190254

Figure 6. Effect of prenatal cold stress on the metabolic and structural changes of PVN neurons in adult rat offspring at 12 weeks of age.

Figure 6.

(A,B) MRS values of adult prenatal cold-exposed and control offspring. The ratios of NAA/Cr (A) and Cho/Cr (B) of the PVN region were acquired by the MRS to detect the central SNS disorder (*P<0.05 vs control, n=6). (C) The intensity of inhibitory and excitatory neurons changes in adult prenatal cold-exposed and control offspring. The excitatory glutamatergic neurons were identified by GRIN2B staining and the inhibitory GABAergic neurons were identified by GAD65 staining. The number of positive cells (black arrow) was counted in ten random fields of the PVN zone from each rat. (*P<0.05 vs control, scale bar = 100 μm, n=4/group).