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. 2019 Oct 22;20(20):5225. doi: 10.3390/ijms20205225

Figure 2.

Figure 2

Responsiveness of copper-related factors. (A,B) HeLa cells were incubated for 16 h with the indicated concentrations of copper sulfate. (A) Western blot analysis of FAS, p53, p21, cyclin D1, ATF3, and c-Fos protein expression. (B) RT-PCR analysis for FAS, p53, p21, cyclin D1, ATF3, and c-Fos mRNA expression. ACTN was the protein loading control; GAPDH mRNA was the mRNA loading control. The results are representative of three independent experiments. Protein and PCR bands were quantified through pixel density scanning and evaluated using ImageJ, version 1.44a (http://imagej.nih.gov/ij/). The fold (shown above the bands) was normalized to the internal control protein (ACTN) or gene (GAPDH).