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Effects of copper sulfate on the ROS, senescence, and mitochondrial membrane potential. (A–C) HeLa cells were incubated with the indicated concentrations of copper sulfate for 16 h in the presence of 5 mM NAC or 5 μM Mito-TEMPO, after which the live cells was stained with (A) 10 μM DCFH-DA; (B) 33 μM C12FDG; or (C) JC-1 and assayed using a flow cytometer. The results are representative of three independent experiments. # p > 0.05, * p < 0.05, ** p < 0.01, and *** p < 0.001.
