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. 2019 Oct 11;20(20):5035. doi: 10.3390/ijms20205035

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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AD significantly decreased neutrophil infiltration. (A,B) Immunohistochemical analysis was performed to detect myeloperoxidase (MPO) (A) and neutrophil elastase (NE) (B) expression in the ankle joint tissue sections of each treatment group on day 37 (n = 10). Representative images are shown. (C,D) The air pouch assay showed that the AD (25 μM) treatment significantly suppressed lipopolysaccharide (LPS)-induced recruitment of total leucocytes (C) and neutrophils (D). The numbers of neutrophils and total leukocytes in the air pouch are expressed as means ± SD. ** p < 0.01 (n ≥ 5).