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. 2019 Oct 11;20(20):5035. doi: 10.3390/ijms20205035

Figure 3.

Figure 3

AD accelerates neutrophil apoptosis in the presence of LPS. Neutrophils were stimulated with LPS (10 ng/mL) or LPS + AD (25 μM) for 16 h, and then the cells were collected for flow cytometry or Western blot analysis. (A) The effects of AD on neutrophil apoptosis were analyzed using flow cytometry. (B) Western blot analyses of B cell lymphoma 2 (Bcl-2), Bcl-2-associated X (Bax), and caspase-3. These experiments were repeated three times and data are expressed as the mean ± SD. * p < 0.05, ** p < 0.01.