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. 2019 Oct 9;20(20):4977. doi: 10.3390/ijms20204977

Figure 2.

Figure 2

Time-course and dose-response of iberin (A) and phenethyl-isothiocyanate (B) treatment effects on proliferation of PC-3 (black pillar) and DU 145 (grey pillar) cells as determined by trypan blue dye exclusion assay. Prostate cancer cells were plated, allowed to attach overnight, and treated with DMSO (control) or desired concentration of iberin (IB), phenethyl-isothiocyanate (PEITC) and/or docetaxel (DOCE) for specified time intervals. Both floating and adherent cells were collected and used for counting of dead and live cells. Data are shown as the mean ± SD of three independent experiments. a, p < 0.05, ITC treatment alone significantly different compared with control treatment; b, p < 0.05, ITC treatment alone for 48 or 72 h significantly different compared with ITC treatment alone for 24 h; c, p < 0.05, ITC treatment alone for 72 h significantly different compared with ITC treatment alone for 48 h; d, p < 0.05, ITC treatment alone significantly different compared with 2 nM DOCE alone; e, p < 0.05, combination treatment (ITC + DOCE 1nM) significantly different compared with 1 nM DOCE alone; f, p < 0.05, combination treatment (ITC + DOCE 2nM) significantly different compared with 2 nM DOCE alone (one-way ANOVA).