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. 2019 Oct 9;20(20):4977. doi: 10.3390/ijms20204977

Table 2.

Sequences of the primers used in reverse transcription-quantitative PCR assays.

Gen/Gene-Related Mechanism Primes RT-qPCR (5′–3′)
MRP1 Drug-transporter gene F: TGTGGACGCTCAGAGGTTCA
R: CATCGCCATCACAGCATTG
CYP3A4
Drug-metabolism gene
Migration gen
F: GGGAAGCAGAGACAGGCAA
R: AAGGGGTCTTGTGGATTGTTG
Topo IIα Target genes F: ATTCAGAGGGGATATGATTCGG
R: GGTTAAATACCAAAGGGGCATA
Cell cycle related genes/apoptosis- related genes Bax F: AGGATGCGTCCACCAAGAAG
R: TGAAGTTGCCGTCAGAAAACA
Bcl-2 F: ATGTGTGTGGAGAGCGTCAACC
R: TGAGCAGAGTCTTCAGAGACAGCC
p21 F: CCCGTGAGCGATGGAACT
R: CGAGGCACAAGGGTACAAGA
GAPDH F: GAAGACTGTGGATGGCCCCTC
R: GTTGAGGGCAATGCCAGCCCC