Table 2.
Gen/Gene-Related Mechanism | Primes RT-qPCR (5′–3′) | |
---|---|---|
MRP1 Drug-transporter gene | F: TGTGGACGCTCAGAGGTTCA R: CATCGCCATCACAGCATTG |
|
CYP3A4 Drug-metabolism gene Migration gen |
F: GGGAAGCAGAGACAGGCAA R: AAGGGGTCTTGTGGATTGTTG |
|
Topo IIα Target genes | F: ATTCAGAGGGGATATGATTCGG R: GGTTAAATACCAAAGGGGCATA |
|
Cell cycle related genes/apoptosis- related genes | Bax | F: AGGATGCGTCCACCAAGAAG R: TGAAGTTGCCGTCAGAAAACA |
Bcl-2 | F: ATGTGTGTGGAGAGCGTCAACC R: TGAGCAGAGTCTTCAGAGACAGCC |
|
p21 | F: CCCGTGAGCGATGGAACT R: CGAGGCACAAGGGTACAAGA |
|
GAPDH | F: GAAGACTGTGGATGGCCCCTC R: GTTGAGGGCAATGCCAGCCCC |