Table A1.
Analysis of synergy between AITC and DOCE calculated by survival rate for PC-3 cells.
| Docetaxel | AITC | Combination Treatment | Index 5 | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Dose | MSR 1 | p2,* | Dose | MSR 1 | p2,* | Expected 3 | Observed 4 | p 2,♦ | ||
| 24 h | 1 | 0.99 | >0.05 | 5 | 0.9 | <0.05 | 0.89 | 0.82 | >0.05 | 1.09 |
| 1 | 0.99 | >0.05 | 10 | 0.78 | <0.05 | 0.77 | 0.6 | <0.05 | 1.28 | |
| 1 | 0.99 | >0.05 | 15 | 0.71 | <0.05 | 0.7 | 0.44 | <0.05 | 1.59 | |
| 1 | 0.99 | >0.05 | 20 | 0.66 | <0.05 | 0.65 | 0.37 | <0.05 | 1.76 | |
| 2 | 0.75 | <0.05 | 5 | 0.9 | <0.05 | 0.68 | 0.65 | >0.05 | 1.05 | |
| 2 | 0.75 | <0.05 | 10 | 0.78 | <0.05 | 0.59 | 0.52 | >0.05 | 1.13 | |
| 2 | 0.75 | <0.05 | 15 | 0.71 | <0.05 | 0.53 | 0.46 | <0.05 | 1.15 | |
| 2 | 0.75 | <0.05 | 20 | 0.66 | <0.05 | 0.5 | 0.34 | <0.05 | 1.47 | |
| 48 h | 1 | 0.95 | >0.05 | 5 | 0.86 | <0.05 | 0.82 | 0.74 | >0.05 | 1.11 |
| 1 | 0.95 | >0.05 | 10 | 0.69 | <0.05 | 0.66 | 0.53 | <0.05 | 1.25 | |
| 1 | 0.95 | >0.05 | 15 | 0.62 | <0.05 | 0.59 | 0.37 | <0.05 | 1.59 | |
| 1 | 0.95 | >0.05 | 20 | 0.57 | <0.05 | 0.54 | 0.31 | <0.05 | 1.74 | |
| 2 | 0.76 | <0.05 | 5 | 0.86 | <0.05 | 0.65 | 0.59 | >0.05 | 1.1 | |
| 2 | 0.76 | <0.05 | 10 | 0.69 | <0.05 | 0.52 | 0.46 | >0.05 | 1.13 | |
| 2 | 0.76 | <0.05 | 15 | 0.62 | <0.05 | 0.47 | 0.35 | <0.05 | 1.34 | |
| 2 | 0.76 | <0.05 | 20 | 0.57 | <0.05 | 0.43 | 0.32 | <0.05 | 1.34 | |
| 72 h | 1 | 0.95 | >0.05 | 5 | 0.74 | <0.05 | 0.7 | 0.69 | >0.05 | 1.01 |
| 1 | 0.95 | >0.05 | 10 | 0.6 | <0.05 | 0.57 | 0.46 | <0.05 | 1.24 | |
| 1 | 0.95 | >0.05 | 15 | 0.56 | <0.05 | 0.53 | 0.31 | <0.05 | 1.71 | |
| 1 | 0.95 | >0.05 | 20 | 0.56 | <0.05 | 0.53 | 0.27 | <0.05 | 1.96 | |
| 2 | 0.66 | <0.05 | 5 | 0.74 | <0.05 | 0.49 | 0.48 | >0.05 | 1.02 | |
| 2 | 0.66 | <0.05 | 10 | 0.6 | <0.05 | 0.4 | 0.4 | >0.05 | 1 | |
| 2 | 0.66 | <0.05 | 15 | 0.56 | <0.05 | 0.37 | 0.35 | >0.05 | 1.06 | |
| 2 | 0.66 | <0.05 | 20 | 0.56 | <0.05 | 0.37 | 0.33 | >0.05 | 1.12 | |
Abbreviations: AITC, allyl isothiocyanate; DMSO, dimethyl sulfoxide; DOCE, docetaxel; MSR, Mean survival rate. 1 MSR of treated group/DMSO-treated control group. 2 p value was determined by one-way ANOVA followed by Tukey’s test. 3 Survival rate of DOCE group multiplied by survival rate of the AITC group. 4 Survival rate of combination treatment group (DOCE + AITC). 5 Index was calculated by dividing the expected survival rate by the observed survival rate. An index of >1 indicates synergistic effect and an index of <1 indicates less than additive effect. * p < 0.05, significantly different compared with control treatment. ♦ p < 0.05, comparison between expected and observed survival rate.