Table A3.

Analysis of synergy between SFN and DOCE calculated by survival rate for PC-3 cells.

	Docetaxel			SFN			Combination Treatment			Index 5
	Dose	MSR 1	p2,*	Dose	MSR 1	p2,*	Expected 3	Observed 4	p 2,♦
24 h	1	0.99	>0.05	15	0.86	<0.05	0.85	0.87	>0.05	0.98
	1	0.99	>0.05	20	0.85	<0.05	0.84	0.86	>0.05	0.98
	1	0.99	>0.05	30	0.81	<0.05	0.8	0.86	>0.05	0.93
	2	0.75	<0.05	15	0.86	<0.05	0.65	0.67	>0.05	0.97
	2	0.75	<0.05	20	0.85	<0.05	0.64	0.67	>0.05	0.96
	2	0.75	<0.05	30	0.81	<0.05	0.61	0.65	>0.05	0.94
48 h	1	0.95	>0.05	15	0.76	<0.05	0.72	0.74	>0.05	0.97
	1	0.95	>0.05	20	0.72	<0.05	0.68	0.74	>0.05	0.92
	1	0.95	>0.05	30	0.72	<0.05	0.68	0.73	>0.05	0.93
	2	0.76	<0.05	15	0.76	<0.05	0.58	0.64	<0.05	0.91
	2	0.76	<0.05	20	0.72	<0.05	0.55	0.62	<0.05	0.89
72 h	1	0.95	>0.05	15	0.72	<0.05	0.68	0.68	>0.05	1
	1	0.95	>0.05	20	0.61	<0.05	0.58	0.62	>0.05	0.94
	1	0.95	>0.05	30	0.59	<0.05	0.56	0.61	>0.05	0.92
	2	0.66	<0.05	15	0.72	<0.05	0.48	0.45	>0.05	1.07
	2	0.66	<0.05	20	0.61	<0.05	0.4	0.39	>0.05	1.03
	2	0.66	<0.05	30	0.59	<0.05	0.39	0.38	>0.05	1.03

Abbreviations: DMSO, dimethyl sulfoxide; DOCE, docetaxel; MSR, mean survival rate; SFN, sulforaphane. ¹ MSR of treated group/DMSO-treated control group. ² p value was determined by one-way ANOVA followed by Tukey’s test. ³ Survival rate of DOCE group multiplied by survival rate of the SFN group. ⁴ Survival rate of combination treatment group (DOCE + SFN). ⁵ Index was calculated by dividing the expected survival rate by the observed survival rate. An index of >1 indicates synergistic effect and an index of <1 indicates less than additive effect. * p < 0.05, significantly different compared with control treatment. ^♦ p < 0.05, comparison between expected and observed survival rate.