Table A4.

Analysis of synergy between SFN and DOCE calculated by survival rate for DU 145 cells.

	Docetaxel			SFN			Combination Treatment			Index 5
	Dose	MSR 1	p2,*	Dose	MSR 1	p2,*	Observed 4	MSR 1	p 2,♦
24 h	1	0.98	>0.05	15	0.92	>0.05	0.9	0.92	>0.05	0.98
	1	0.98	>0.05	20	0.85	<0.05	0.83	0.83	>0.05	1
	1	0.98	>0.05	30	0.85	<0.05	0.83	0.84	>0.05	0.99
	2	0.75	<0.05	15	0.92	>0.05	0.69	0.79	<0.05	0.87
	2	0.75	<0.05	20	0.85	<0.05	0.64	0.65	>0.05	0.98
	2	0.75	<0.05	30	0.85	<0.05	0.64	0.65	>0.05	0.98
48 h	1	1	>0.05	15	0.71	<0.05	0.71	0.69	>0.05	1.03
	1	1	>0.05	20	0.71	<0.05	0.71	0.69	>0.05	1.03
	1	1	>0.05	30	0.7	<0.05	0.7	0.64	>0.05	1.09
	2	0.77	<0.05	15	0.71	<0.05	0.55	0.22	<0.05	2.5
	2	0.77	<0.05	20	0.71	<0.05	0.55	0.2	<0.05	2.75
72 h	1	0.98	>0.05	15	0.43	<0.05	0.42	0.37	>0.05	1.14
	1	0.98	>0.05	20	0.43	<0.05	0.42	0.36	>0.05	1.17
	1	0.98	>0.05	30	0.4	<0.05	0.39	0.33	>0.05	1.18
	2	0.68	<0.05	15	0.43	<0.05	0.29	0.23	>0.05	1.26
	2	0.68	<0.05	20	0.43	<0.05	0.29	0.21	>0.05	1.38
	2	0.68	<0.05	30	0.4	<0.05	0.27	0.2	>0.05	1.35

Abbreviations: DMSO, dimethyl sulfoxide; DOCE, docetaxel; MSR, mean survival rate; SFN, sulforaphane. ¹ MSR of treated group/DMSO-treated control group. ² p value was determined by one-way ANOVA followed by Tukey’s test. ³ Survival rate of DOCE group multiplied by survival rate of the SFN group. ⁴ Survival rate of combination treatment group (DOCE + SFN). ⁵ Index was calculated by dividing the expected survival rate by the observed survival rate. An index of >1 indicates synergistic effect and an index of <1 indicates less than additive effect. * p < 0.05, significantly different compared with control treatment. ^♦ p < 0.05, comparison between expected and observed survival rate.