Table A6.

Analysis of synergy between IB and DOCE calculated by survival rate for DU 145 cells.

	Docetaxel			IB			Combination Treatment			Index 5
	Dose	MSR 1	p2,*	Dose	MSR 1	p2,*	Expected 3	Observed 4	p 2,♦
24 h	1	0.98	>0.05	5	0.61	<0.05	0.6	0.95	<0.05	0.63
	1	0.98	>0.05	15	0.67	<0.05	0.66	0.93	<0.05	0.71
	1	0.98	>0.05	30	0.7	<0.05	0.69	0.9	<0.05	0.77
	2	0.75	<0.05	5	0.61	<0.05	0.46	0.79	<0.05	0.58
	2	0.75	<0.05	15	0.67	<0.05	0.5	0.73	<0.05	0.68
	2	0.75	<0.05	30	0.7	<0.05	0.53	0.77	<0.05	0.69
48 h	1	1	>0.05	5	0.75	<0.05	0.75	0.92	<0.05	0.82
	1	1	>0.05	15	0.79	<0.05	0.79	0.95	<0.05	0.83
	1	1	>0.05	30	0.72	<0.05	0.72	0.51	<0.05	1.41
	2	0.77	<0.05	5	0.75	<0.05	0.58	0.81	<0.05	0.72
	2	0.77	<0.05	15	0.79	<0.05	0.61	0.74	<0.05	0.82
	2	0.77	<0.05	30	0.72	<0.05	0.55	0.7	<0.05	0.79
72 h	1	0.98	>0.05	5	0.93	>0.05	0.91	0.74	<0.05	1.23
	1	0.98	>0.05	15	0.84	<0.05	0.82	0.41	<0.05	2
	1	0.98	>0.05	30	0.4	<0.05	0.39	0.32	>0.05	1.22
	2	0.68	<0.05	5	0.93	>0.05	0.63	0.58	>0.05	1.09
	2	0.68	<0.05	15	0.84	<0.05	0.57	0.55	>0.05	1.04
	2	0.68	<0.05	30	0.4	<0.05	0.27	0.47	<0.05	0.57

Abbreviations: DMSO, dimethyl sulfoxide; DOCE, docetaxel; IB, iberin; MSR, mean survival rate. ¹ MSR, of treated group/DMSO-treated control group. ² p value was determined by one-way ANOVA followed by Tukey’s test. ³ Survival rate of DOCE group multiplied by survival rate of the IB group. ⁴ Survival rate of combination treatment group (DOCE + IB). ⁵ Index was calculated by dividing the expected survival rate by the observed survival rate. An index of >1 indicates synergistic effect and an index of <1 indicates less than additive effect. * p < 0.05, significantly different compared with control treatment. ^♦ p < 0.05, comparison between expected and observed survival rate.