Table A8.

Analysis of synergy between PEITC and DOCE calculated by survival rate for DU 145 cells.

	Docetaxel			PEITC			Combination Treatment			Index 5
	Dose	MSR 1	p2,*	Dose	MSR 1	p2,*	Expected 3	Observed 4	p 2,♦
24 h	1	0.98	>0.05	1	0.9	<0.05	0.88	0.87	>0.05	1.01
	1	0.98	>0.05	2	0.72	<0.05	0.71	0.67	>0.05	1.06
	1	0.98	>0.05	4	0.45	<0.05	0.44	0.44	>0.05	1
	2	0.75	<0.05	1	0.9	<0.05	0.68	0.6	>0.05	1.13
	2	0.75	<0.05	2	0.72	<0.05	0.54	0.46	<0.05	1.17
	2	0.75	<0.05	4	0.45	<0.05	0.34	0.33	>0.05	1.03
48 h	1	1	>0.05	1	0.85	<0.05	0.85	0.84	>0.05	1.01
	1	1	>0.05	2	0.66	<0.05	0.66	0.61	>0.05	1.08
	1	1	>0.05	4	0.48	<0.05	0.48	0.4	>0.05	1.2
	2	0.77	<0.05	1	0.85	<0.05	0.65	0.61	>0.05	1.07
	2	0.77	<0.05	2	0.66	<0.05	0.51	0.48	>0.05	1.06
	2	0.77	<0.05	4	0.48	<0.05	0.37	0.35	>0.05	1.06
72 h	1	0.98	>0.05	1	0.82	<0.05	0.8	0.8	>0.05	1
	1	0.98	>0.05	2	0.67	<0.05	0.66	0.58	<0.05	1.14
	1	0.98	>0.05	4	0.45	<0.05	0.44	0.44	>0.05	1
	2	0.68	<0.05	1	0.82	<0.05	0.56	0.54	>0.05	1.04
	2	0.68	<0.05	2	0.67	<0.05	0.46	0.42	>0.05	1.1
	2	0.68	<0.05	4	0.45	<0.05	0.31	0.3	>0.05	1.03

Abbreviations: DMSO, dimethyl sulfoxide; DOCE, docetaxel; MSR, mean survival rate; PEITC, phenethyl-ITC. ¹ MSR, of treated group/DMSO-treated control group. ² p value was determined by one-way ANOVA followed by Tukey’s test. ³ Survival rate of DOCE group multiplied by survival rate of the PEITC group. ⁴ Survival rate of combination treatment group (DOCE + PEITC). ⁵ Index was calculated by dividing the expected survival rate by the observed survival rate. An index of >1 indicates synergistic effect and an index of <1 indicates less than additive effect. * p < 0.05, significantly different compared with control treatment. ^♦ p < 0.05, comparison between expected and observed survival rate.